Lifesign 1000 - Uricult CLED/EMB (Urinary Tract Infections) 1000 MTC, 10/Bx, 50 Bx/Cs


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Description

Product Description

Lifesign 1000 - Uricult CLED/EMB (Urinary Tract Infections) 1000 MTC, 10/Bx, 50 Bx/Cs

Uricult CLED/EMB (Urinary Tract Infections)

10 Urine Culture-Paddles for the detection of bacteriuria and the presumptive identification of uropathogens. The test is intended for in vitro diagnostic use.

  • Moderately Complex
  • Culture method for urinary pathogens
  • Easy to perform procedure; Dip - Incubate - Read Results
  • Quick and convenient result within 24 hours
  • Simple CLIA compliance for reporting colony counts.

Summary and explanation of the test

URICULT Urine CULTURE-PADDLES provide effective bacterial detection and presumptive identification in a simple and reliable manner. URICULT Urine CULTURE-PADDLES are attached to a screw cap. Each side of the culture-paddle is coated with an agar medium suitable for the growth of urinary bacteria, and the culture-paddle is suspended in a clear plastic vial.

The CULTURE-PADDLES are safely isolated in this vial during transport, incubation, storage and handling. Because of the uniform application of agar to the URICULT Urine CULTUREPADDLE, it is possible to obtain semi-quantitative results when the device is used as directed. This is determined by a simple visual comparison of bacterial growth on the agar surface with the Colony Density Chart provided. No actual colony counting is necessary.

Principles of the procedure

CLED Agar (Cystine-Lactose-Electrolyte-Deficient) CLED medium was first described by Mackey and Sandys1 specifically for use in dip inoculum procedures for urinary bacteriology. This agar supports the growth of both Gram positive and Gram negative bacteria commonly encountered in urine specimens. The electrolyte deficient nature of the medium prevents the characteristics swarming of Proteus. The inclusion of lactose and the indicator dye, Brom-Thymol Blue, allows differentiation of lactose fermenting bacteria, since the by-products of lactose fermentation cause the color of the agar to change from its original pale green color toward yellow.

EMB Agar (Eosin Methylene Blue) EMB agar is a selective reddish-brown colored medium recommended for the detection and isolation of Gram negative intestinal pathogenic bacteria. The balanced combination of the two dyes (Eosin and Methylene Blue) contained in the EMB agar makes it possible to distinguish lactose fermenting bacteria from non-fermenters. Those bacteria which ferment lactose will have dark colonies, purple or black in color. A green sheen may be observed across the agar surface during the growth of certain lactose fermenting bacteria.

Reagents

Uricult CLED/EMB Product No. 1000
Urine CULTURE-PADDLES (in individual vials) 10
Patient labels I 10
Instructions for use containing the Colony Density Chart 1

Typical formulation

CLED medium EMB medium
Peptone 10.0 g/L Peptone 10.0 g/L
Meat extract 3.0 g/L Lactose 5.0 g/L
Lactose 10.0 g/L Sucrose 5.0 g/L
L-Cystine 0.13 g/L Dipotassium
Brom-Thymol Blue Phosphate 2.0 g/L
Eosin Y
Methylene Blue

Colony Density Chart

LifeSign Uricult CLED/EMB 1000

Procedure

Compliance with the following directions is required to achieve reliable test results.

LifeSign Uricult CLED/EMB 1000

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